Long-Read cDNA Sequencing Revealed Novel Expressed Genes and Dynamic Transcriptome Landscape of Triticale (x Triticosecale Wittmack) Seed at Different Developing Stages

Developing seed is a unique stage of plant development with highly dynamic changes in transcriptome. Here, we aimed to detect the novel previously unannotated, genes triticale (x Triticosecale Wittmack, AABBRR genome constitution) that are expressed during different stages and at parts developing seed. For this, carried out Oxford Nanopore sequencing cDNA obtained for middle (15 days post-anthesis, dpa) late (20 development. The data together our previous direct RNA early (10 revealed 39,914 including 7128 (17.6%) were not annotated A, B, R genomes. bioinformatic analysis showed identified belonged long non-coding RNAs (lncRNAs), protein-coding RNAs, TE-derived RNAs. gene set transcriptome dynamics distinct patterns over-represented functions middle/late stages. We performed lncRNA polymorphism some tested lncRNAs indeed polymorphic collection. Altogether, results provide information on thousands loci can be used as new targets GWAS analysis, marker-assisted breeding triticale, functional elucidation.